Various lysates were subjected to SDS PAGE followed by western blot with 16743-1-AP (PHKG1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16743-1-AP (PHKG1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 16743-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16743-1-AP (PHKG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16743-1-AP (PHKG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 16743-1-AP
IP result of anti-PHKG1 (IP:16743-1-AP, 4ug; Detection:16743-1-AP 1:600) with mouse skeletal muscle tissue lysate 4000ug.
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mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected in
mouse skeletal muscle tissue
Positive IHC detected in
mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:5000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PHKG1(Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform) is also named as phosphorylase kinase subunit gamma-1, PHKG and belongs to the CAMK Ser/Thr protein kinase family. Phosphorylase kinase is a crucial glycogenolytic regulatory enzyme. The holoenzymes are composed of 16 subunit proteins containing equimolar ratios of 4 subunit types known as alpha, beta, gamma, and delta. This protein is the skeletal muscle isoform of phosphorylase kinase and kinetic differences exist between the cardiac and adult skeletal muscle enzymes, and the liver and skeletal muscle enzymes are known to be separate isozymes.
Various lysates were subjected to SDS PAGE followed by western blot with 16743-1-AP (PHKG1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 16743-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16743-1-AP (PHKG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse skeletal muscle using 16743-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16743-1-AP (PHKG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 16743-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16743-1-AP (PHKG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse skeletal muscle using 16743-1-AP
IP result of anti-PHKG1 (IP:16743-1-AP, 4ug; Detection:16743-1-AP 1:600) with mouse skeletal muscle tissue lysate 4000ug.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PHKG1 Polyclonal antibody
Catalog Number
16743-1-AP
Citations
2
Dilutions
WB : 1:1000-1:5000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500
Applications
WB, IP, IHC, ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
