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Twinkle/PEO1 Polyclonal antibody

Twinkle/PEO1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18793-1-AP

Synonyms

ATXN8, C10orf2, IOSCA, PEO, PEO1, PEOA3, SANDO, SCA8, Twinkle, Twinkle protein, mitochondrial, TWINL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Twinkle/PEO1 Polyclonal antibody (18793-1-AP)

    WB analysis using 18793-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18793-1-AP (Twinkle antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Twinkle/PEO1 Polyclonal antibody (18793-1-AP)

    IHC staining of human pancreas using 18793-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Twinkle/PEO1 Polyclonal antibody (18793-1-AP)

    IHC staining of human pancreas using 18793-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Twinkle/PEO1 Polyclonal antibody (18793-1-AP)

    IHC staining of human skeletal muscle using 18793-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Twinkle/PEO1 Polyclonal antibody (18793-1-AP)

    IHC staining of human skeletal muscle using 18793-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inDaudi cells, MCF-7 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18793-1-AP targets Twinkle/PEO1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Twinkle/PEO1 fusion protein Ag6628
Full Name chromosome 10 open reading frame 2
Calculated Molecular Weight 684 aa, 77 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC033762
Gene Symbol C10orf2
Gene ID (NCBI) 56652
RRIDAB_2066354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Twinkle, also named as Progressive external ophthalmoplegia 1 protein is a 684 amino acid protein, which involves in mitochondrial DNA (mtDNA) metabolism. The predicted full-length protein is 684 amino acids, with amolecular mass of 77 kD, whereas the splice variant mRNAencodes a 66-kD product of 582 amino acids, lacking residues 579-684 and terminating with four unique amino acids (PMID: 11431692).

Protocols

Product Specific Protocols
WB protocol for Twinkle/PEO1 antibody 18793-1-APDownload protocol
IHC protocol for Twinkle/PEO1 antibody 18793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols