- Featured Product
- KD/KO Validated
PDK2 Polyclonal antibody
PDK2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 15647-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human heart tissue, HeLa cells, mouse skeletal muscle tissue, NCI-H1299 cells, rat heart tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue, human testis tissue, human placenta tissue, human kidney tissue, human heart tissue, human liver tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
Product Information
15647-1-AP targets PDK2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDK2 fusion protein Ag8183 |
| Full Name | pyruvate dehydrogenase kinase, isozyme 2 |
| Calculated Molecular Weight | 407 aa, 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC005811 |
| Gene Symbol | PDK2 |
| Gene ID (NCBI) | 5164 |
| RRID | AB_2268006 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDK2([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial) is also named as PDHK2 and belongs to the PDK/BCKDK protein kinase family. PDK2 plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDK2 is also involved in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDK2 antibody 15647-1-AP | Download protocol |
| IHC protocol for PDK2 antibody 15647-1-AP | Download protocol |
| IP protocol for PDK2 antibody 15647-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Transl Med Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice. | ||
Hepatology Retinoic acid-related orphan receptor alpha reprograms glucose metabolism in glutamine-deficient hepatoma cells. | ||
Oncogene Dichloroacetate restores colorectal cancer chemosensitivity through the p53/miR-149-3p/PDK2-mediated glucose metabolic pathway. | ||
Mol Ther Oncolytics ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer. | ||
Sci Rep Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells. | ||
Am J Physiol Lung Cell Mol Physiol Contribution of Fatty Acid Oxidation to the Pathogenesis of Pulmonary Hypertension. |
