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PDF Polyclonal antibody

PDF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24842-1-AP

Synonyms

PDF, PDF1A, Polypeptide deformylase

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PDF Polyclonal antibody (24842-1-AP)

    WB analysis of MCF-7 using 24842-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using PDF Polyclonal antibody (24842-1-AP)

    WB analysis of MDA-MB-453s using 24842-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using PDF Polyclonal antibody (24842-1-AP)

    WB analysis of MDA-MB-453s using 24842-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDF Polyclonal antibody (24842-1-AP)

    IHC staining of human breast cancer using 24842-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24842-1-AP (PDF Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDF Polyclonal antibody (24842-1-AP)

    IHC staining of human breast cancer using 24842-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24842-1-AP (PDF Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24842-1-AP is PDF Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDF fusion protein Ag18233
Full Name peptide deformylase (mitochondrial)
Calculated Molecular Weight 243 aa, 27 kDa
Observed Molecular Weight 60 kDa
GenBank Accession Numberbc019912
Gene Symbol PDF
Gene ID (NCBI) 64146
RRIDAB_2879753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDF antibody 24842-1-APDownload protocol
IHC protocol for PDF antibody 24842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols