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PDE9A Polyclonal antibody

PDE9A Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12648-1-AP

Synonyms

HSPDE9A2, High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A, EC:3.1.4.35

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PDE9A Polyclonal antibody (12648-1-AP)

    IHC staining of human prostate cancer using 12648-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12648-1-AP (PDE9A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PDE9A Polyclonal antibody (12648-1-AP)

    IHC staining of human prostate cancer using 12648-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12648-1-AP (PDE9A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PDE9A Polyclonal antibody (12648-1-AP)

    IHC staining of human prostate cancer using 12648-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12648-1-AP (PDE9A antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PDE9A Polyclonal antibody (12648-1-AP)

    IF Staining of A549 using 12648-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 12648-1-AP (PDE9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12648-1-AP targets PDE9A in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE9A fusion protein Ag3372
Full Name phosphodiesterase 9A
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC009047
Gene Symbol PDE9A
Gene ID (NCBI) 5152
RRIDAB_2161890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclic nucleotide phosphodiesterases (PDEs), which hydrolyze the intracellular second messengers cAMP and cGMP to their corresponding monophosphates, play an important role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE9A plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. This protein has 16 isoforms with the MW between 44 kDa and 65 kDa.

Protocols

Product Specific Protocols
IHC protocol for PDE9A antibody 12648-1-APDownload protocol
IF protocol for PDE9A antibody 12648-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,rat

Eur J Pharmacol

A novel phosphodiesterase 9A inhibitor LW33 protects against ischemic stroke through the cGMP/PKG/CREB pathway.

Authors - Jia-Yi You
View Article