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PDE12 Polyclonal antibody

PDE12 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 12753-1-AP

Synonyms

2'-PDE, 2'PDE, 2PDE, 2',5'-phosphodiesterase 12, 2' PDE

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using PDE12 Polyclonal antibody (12753-1-AP)

    WB analysis of human brain using 12753-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDE12 Polyclonal antibody (12753-1-AP)

    WB analysis using 12753-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PDE12 Polyclonal antibody (12753-1-AP)

    IP experiment of mouse brain using 12753-1-AP

    IP result of anti-PDE12 (IP:12753-1-AP, 4ug; Detection:12753-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDE12 Polyclonal antibody (12753-1-AP)

    IF Staining of HepG2 using 12753-1-AP

    Immunofluorescent analysis of HepG2 cells, using PDE12 antibody 12753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using PDE12 Polyclonal antibody (12753-1-AP)

    FC experiment of HepG2 using 12753-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug PDE12 Polyclonal antibody (12753-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman brain tissue, THP-1 cells
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12753-1-AP targets PDE12 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE12 fusion protein Ag3217
Full Name phosphodiesterase 12
Calculated Molecular Weight 472 aa, 52 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC034978
Gene Symbol PDE12
Gene ID (NCBI) 201626
RRIDAB_2162883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDE12 antibody 12753-1-APDownload protocol
IF protocol for PDE12 antibody 12753-1-APDownload protocol
IP protocol for PDE12 antibody 12753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Eur J Pharmacol

PDE12 disrupts mitochondrial oxidative phosphorylation and mediates mitochondrial dysfunction to induce oral mucosal epithelial barrier damage in oral submucous fibrosis

Authors - Qihui Wei
  • KD Validated
View Article