PDE10A Polyclonal antibody
PDE10A Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 18078-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
18078-1-AP targets PDE10A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDE10A fusion protein Ag12771 |
| Full Name | phosphodiesterase 10A |
| Calculated Molecular Weight | 779 aa, 88 kDa |
| Observed Molecular Weight | 89 kDa |
| GenBank Accession Number | BC104860 |
| Gene Symbol | PDE10A |
| Gene ID (NCBI) | 10846 |
| RRID | AB_2878492 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDE10A antibody 18078-1-AP | Download protocol |
| IF protocol for PDE10A antibody 18078-1-AP | Download protocol |
| IP protocol for PDE10A antibody 18078-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Environ Int Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms | ||
EMBO J A genetically targeted reporter for PET imaging of deep neuronal circuits in mammalian brains. | ||
Andrology Papaverine-sensitive phosphodiesterase activity is measured in bovine spermatozoa. |
