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PDCL3 Polyclonal antibody

PDCL3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14997-1-AP

Synonyms

Phosducin-like protein 3, Phosducin like protein 3, phosducin like 3, PHLP3, PhLP2A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PDCL3 Polyclonal antibody (14997-1-AP)

    WB analysis using 14997-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14997-1-AP (PDCL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDCL3 Polyclonal antibody (14997-1-AP)

    WB analysis using 14997-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14997-1-AP (PDCL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDCL3 Polyclonal antibody (14997-1-AP)

    IF Staining of HepG2 using 14997-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDCL3 antibody (14997-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PDCL3 Polyclonal antibody (14997-1-AP)

    IF Staining of MCF-7 using 14997-1-AP

    Immunofluorescent analysis of MCF-7 cells, using PDCL3 antibody 14997-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PDCL3 Polyclonal antibody (14997-1-AP)

    IF Staining of HeLa using 14997-1-AP

    Immunofluorescent analysis of Hela cells, using PDCL3 antibody 14997-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA2780 cells, COLO 320 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells, MCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14997-1-AP targets PDCL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCL3 fusion protein Ag6983
Full Name phosducin-like 3
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC001021
Gene Symbol PDCL3
Gene ID (NCBI) 79031
RRIDAB_2878098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDCL3 antibody 14997-1-APDownload protocol
IF protocol for PDCL3 antibody 14997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Eur J Med Res

PDCL3 is a prognostic biomarker associated with immune infiltration in hepatocellular carcinoma

Authors - Wenzhi Jin
View Article