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  • Featured Product
  • KD/KO Validated

PCTAIRE1 Polyclonal antibody

PCTAIRE1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10102-1-AP

Synonyms

CDK16, PCTAIRE, PCTAIRE motif protein kinase 1, PCTAIRE protein kinase 1, PCTAIRE1, PCTGAIRE, PCTK1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PCTAIRE1 Polyclonal antibody (10102-1-AP)

    WB analysis of mouse brain using 10102-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10102-1-AP (PCTAIRE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PCTAIRE1 Polyclonal antibody (10102-1-AP)

    WB analysis of mouse brain using 10102-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10102-1-AP (PCTAIRE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PCTAIRE1 Polyclonal antibody (10102-1-AP)

    IP experiment of mouse brain using 10102-1-AP

    IP result of anti-PCTAIRE1 (IP:10102-1-AP, 4ug; Detection:10102-1-AP 1:500) with mouse brain tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PCTAIRE1 Polyclonal antibody (10102-1-AP)

    IHC staining of human pancreas using 10102-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10102-1-AP (PCTAIRE1 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PCTAIRE1 Polyclonal antibody (10102-1-AP)

    IHC staining of human pancreas using 10102-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10102-1-AP (PCTAIRE1 antibody at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10102-1-AP targets PCTAIRE1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCTAIRE1 fusion protein Ag0151
Full Name PCTAIRE protein kinase 1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-63 kDa
GenBank Accession NumberBC001048
Gene Symbol PCTAIRE1
Gene ID (NCBI) 5127
RRIDAB_513483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PCTAIRE1 antibody 10102-1-APDownload protocol
IHC protocol for PCTAIRE1 antibody 10102-1-APDownload protocol
IP protocol for PCTAIRE1 antibody 10102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Theranostics

CDK16 Phosphorylates and Degrades p53 to Promote Radioresistance and Predicts Prognosis in Lung Cancer.

Authors - Jie Xie
View Article
mouseWB

Elife

Loss of Tsc1 in cerebellar Purkinje cells induces transcriptional and translation changes in FMRP target transcripts

Authors - Jasbir Singh Dalal
View Article
humanWB

Mol Oncol

Lung cancer cells expressing a shortened CDK16 3'UTR escape senescence through impaired miR-485-5p targeting.

Authors - Qi Jia
  • KD Validated
View Article
humanWB

Tumour Biol

Functional elucidation of miR-494 in the tumorigenesis of nasopharyngeal carcinoma.

Authors - Hong-Fang Duan
View Article