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PCDH24 Polyclonal antibody

PCDH24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27103-1-AP

Synonyms

CDHR2, PC LKC, PCDH24, PCLKC, protocadherin 24, Protocadherin LKC

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    WB analysis of mouse liver using 27103-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27103-1-AP (PCDH24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of human kidney using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of human kidney using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of mouse small intestine using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of mouse small intestine using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of human liver using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of human liver using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of human small intestine using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of human small intestine using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of human colon cancer using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PCDH24 Polyclonal antibody (27103-1-AP)

    IHC staining of human colon cancer using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, human liver tissue, human small intestine tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27103-1-AP is PCDH24 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDH24 fusion protein Ag25840
Full Name protocadherin 24
Calculated Molecular Weight 1310 aa, 142 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC130282
Gene Symbol PCDH24
Gene ID (NCBI) 54825
RRIDAB_2880757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherin-24 (PCDH24, also known as CDHR2 and protocadherin LKC) was originally identified as a novel member of the human non-classical cadherin family. PCDH24 may play a role in cell-cell adhesion and contact inhibition in epithelial cells. PCDH24 expression is markedly reduced in cancers derived from liver and colon tissues, indicating that PCDH24 may play a role as a tumor suppressor protein (PMID: 23115611). The calculated molecular weight of PCDH24 is 142 kDa.

Protocols

Product Specific Protocols
WB protocol for PCDH24 antibody 27103-1-APDownload protocol
IHC protocol for PCDH24 antibody 27103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Transl Androl Urol

Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.

Authors - Xuechao Li
View Article