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PCBD1 Polyclonal antibody

PCBD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15702-1-AP

Synonyms

PCD, PCBD, EC:4.2.1.96, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, DCOH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCBD1 Polyclonal antibody (15702-1-AP)

    WB analysis using 15702-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of mouse liver using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of mouse liver using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCBD1 Polyclonal antibody (15702-1-AP)

    IF Staining of HeLa using 15702-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCBD1 antibody (15702-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15702-1-AP targets PCBD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCBD1 fusion protein Ag8331
Full Name pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
Calculated Molecular Weight 104 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC006324
Gene Symbol PCBD1
Gene ID (NCBI) 5092
RRIDAB_2267945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCBD1, also known as DCOH and PCBD, belongs to the pterin-4-alpha-carbinolamine dehydratase family. PCBD1 is a bifunctional protein that acts as an enzyme in the regeneration of cofactor tetrahydrobiopterin (BH4), crucial for the function of aromatic amino acid hydroxylases, and as a dimerization cofactor of transcription factors HNF1A and HNF1B, important in liver and pancreas development and function. PCBD1 is expressed in the developing pancreas. The calculated molecular weight of PCBD1 is 12 kDa (PMID: 24204001, 24848070).

Protocols

Product Specific Protocols
WB protocol for PCBD1 antibody 15702-1-APDownload protocol
IHC protocol for PCBD1 antibody 15702-1-APDownload protocol
IF protocol for PCBD1 antibody 15702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols