- Featured Product
- KD/KO Validated
PBX3 Polyclonal antibody
PBX3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 12571-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human lung tissue |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| ChIP | See 2 publications below |
| RIP | See 1 publications below |
Product Information
12571-1-AP targets PBX3 in WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PBX3 fusion protein Ag3239 |
| Full Name | pre-B-cell leukemia homeobox 3 |
| Calculated Molecular Weight | 434 aa, 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC016977 |
| Gene Symbol | PBX3 |
| Gene ID (NCBI) | 5090 |
| RRID | AB_2160469 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PBX3, also named as Pre-B-cell leukemia transcription factor 3, is a 434 amino acid protein ,which contains 1 homeobox DNA-binding domain and belongs to the TALE/PBX homeobox family. PBX3 localizes in the nucleus and as a transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. PBX3 is ubiquitously expressed in many tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PBX3 antibody 12571-1-AP | Download protocol |
| IHC protocol for PBX3 antibody 12571-1-AP | Download protocol |
| IF protocol for PBX3 antibody 12571-1-AP | Download protocol |
| IP protocol for PBX3 antibody 12571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis. | ||
Haematologica P2X7 promotes the progression of MLL-AF9 induced acute myeloid leukemia by upregulation of Pbx3.
| ||
Cell Mol Gastroenterol Hepatol scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3. | ||
Free Radic Biol Med Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection. | ||
Int J Biol Sci USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling. | ||
Sci Rep Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu. |
