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PAX8-Specific Monoclonal antibody

PAX8-Specific Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H9E1

Cat no : 66073-1-Ig

Synonyms

paired box 8, Paired box protein Pax 8, PAX8, PAX8-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using PAX8-Specific Monoclonal antibody (66073-1-Ig)

    WB analysis of SKOV-3 using 66073-1-Ig

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66073-1-Ig (PAX8-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSkov-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66073-1-Ig targets PAX8-Specific in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name paired box 8
Calculated Molecular Weight 48 kDa
GenBank Accession NumberNM_013992
Gene Symbol PAX8
Gene ID (NCBI) 7849
RRIDAB_11064732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Several other splice variants have been proposed but the full nature of these products has not been described. Pax8 is also a marker of otic progenitor cells. Mutation of PAX8 will cause congenital hypothyroidism non-goitrous type 2 (CHNG2). This antibody is specific to Pax8. It will not cross react with other PAX members

Protocols

Product Specific Protocols
WB protocol for PAX8-Specific antibody 66073-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols