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PARP9 Polyclonal antibody
PARP9 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17535-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, THP-1 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human spleen tissue, human skin tissue, human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IF | See 2 publications below |
Product Information
17535-1-AP targets PARP9 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, treeshrew |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PARP9 fusion protein Ag11587 |
| Full Name | poly (ADP-ribose) polymerase family, member 9 |
| Calculated Molecular Weight | 819 aa, 92 kDa |
| Observed Molecular Weight | 88 kDa |
| GenBank Accession Number | BC039580 |
| Gene Symbol | PARP9 |
| Gene ID (NCBI) | 83666 |
| RRID | AB_2158929 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by one of the 17 members of the poly(ADP-ribose) polymerases (PARP). PARP-9 belongs to the subfamily of macroPARPs, associating one to three macro domains to the PARP domain. Overexpression of PARP-9 stimulates cell migration in vitro, suggesting a role for PARP-9 in the promotion of malignant B cell migration and dissemination in high risk DLBCL. PARP-9 is also likely a transcription coactivator, its overexpression in B lymphocytes, stimulated by IFNγ, inducing the transcription of IFNγ-controlled genes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PARP9 antibody 17535-1-AP | Download protocol |
| IHC protocol for PARP9 antibody 17535-1-AP | Download protocol |
| IP protocol for PARP9 antibody 17535-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol An Alternative Splicing of Tupaia STING Modulated Anti-RNA Virus Responses by Targeting MDA5-LGP2 and IRF3.
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Oncol Lett PARP9 is overexpressed in human breast cancer and promotes cancer cell migration.
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J Mol Biol KH-like domains in PARP9/DTX3L and PARP14 coordinate protein-protein interactions to promote cancer cell survival
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hadil (Verified Customer) (05-25-2023) | I have had trouble detecting PARP9 signals with other PARP9 antibodies, however, this antibody is a game changer. It works well both at room temperature (1.5 -2 hrs) and also overnight at 1:500 dilution. Would highly recommend to anyone who's studying PARP9.
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