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  • Featured Product
  • KD/KO Validated

PARP1 Monoclonal antibody

PARP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1D7D4

Cat no : 66520-1-Ig

Synonyms

ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis of Jurkat using 66520-1-Ig

    Jurkat cells (20 μg/lane) treated with staurosporine were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis of HSC-T6 using 66520-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis using 66520-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis of RAW 264.7 using 66520-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis using 66520-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis of HeLa using 66520-1-Ig

    HeLa cells (20 μg) were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using PARP1 Monoclonal antibody (66520-1-Ig)

    IP experiment of K-562 using 66520-1-Ig

    IP result of anti-PARP1 (IP:66520-1-Ig, 5ug; Detection:66520-1-Ig 1:10000) with K-562 cells lysate 2760 ug.

  • Immunohistochemistry (IHC) staining of rat colon tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of rat colon using 66520-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of human lung cancer using 66520-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of human lung cancer using 66520-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of mouse testis using 66520-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of mouse colon using 66520-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of human breast cancer using 66520-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of human breast cancer using 66520-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using PARP1 Monoclonal antibody (66520-1-Ig)

    IF Staining of Neuro-2a using 66520-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PARP1 antibody (66520-1-Ig, Clone: 1D7D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using PARP1 Monoclonal antibody (66520-1-Ig)

    FC experiment of HeLa using 66520-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of Jurkat cells using PARP1 Monoclonal antibody (66520-1-Ig)

    FC experiment of Jurkat using 66520-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells, HeLa cells, HSC-T6 cells, HepG2 cells, ROS1728 cells, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, mouse colon tissue, mouse testis tissue, human breast cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66520-1-Ig targets PARP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, zebrafish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PARP1 fusion protein Ag19173
Full Name poly (ADP-ribose) polymerase 1
Calculated Molecular Weight 1014 aa, 113 kDa
Observed Molecular Weight 113-116 kDa, 85-89 kDa
GenBank Accession NumberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
RRIDAB_2881883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Protocols

Product Specific Protocols
WB protocol for PARP1 antibody 66520-1-IgDownload protocol
IHC protocol for PARP1 antibody 66520-1-IgDownload protocol
IF protocol for PARP1 antibody 66520-1-IgDownload protocol
IP protocol for PARP1 antibody 66520-1-IgDownload protocol
FC protocol for PARP1 antibody 66520-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair

Authors - Liu Liu
View Article
humanWB

Nat Chem Biol

Enhanced mapping of small-molecule binding sites in cells

Authors - Jacob M Wozniak
View Article
ratWB,IP

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
View Article
humanIHC

J Exp Clin Cancer Res

PARP1-targeted fluorescence molecular endoscopy as novel tool for early detection of esophageal dysplasia and adenocarcinoma

Authors - Sabrina Marcazzan
View Article
humanWB

Biomaterials

Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling.

Authors - Shaojun Pan
View Article
humanWB

Arthritis Rheumatol

Association of the Polymorphism rs13259960 in SLEAR With Predisposition to Systemic Lupus Erythematosus.

Authors - Zhen Fan
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Rashmi (Verified Customer) (09-25-2024)

Excellent Product

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Vikas (Verified Customer) (07-11-2024)

Used for Immunoblot. Highly recommended product.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y cell line
FH

Hadil (Verified Customer) (05-23-2023)

This antibody is a great choice, delivering strong signals and enhanced specificity. It has low background noise, ensuring accurate results. Its efficiency saves valuable time, making it a reliable and versatile tool for various applications. A must-have for researchers seeking reliable and efficient antibody performance.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: Mammalian cell lines
PARP1 Antibody Western Blot validation (1:10,000 dilution) in Mammalian cell lines (Cat no:66520-1-Ig)
FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other
FH

MANOHAR (Verified Customer) (12-11-2019)

No nonspecific binding

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T