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PAPSS1 Polyclonal antibody

PAPSS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14708-1-AP

Synonyms

ATP sulfurylase, APS kinase, Adenylyl-sulfate kinase, Adenylylsulfate 3'-phosphotransferase, Adenosine-5'-phosphosulfate 3'-phosphotransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PAPSS1 Polyclonal antibody (14708-1-AP)

    WB analysis of HepG2 using 14708-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PAPSS1 Polyclonal antibody (14708-1-AP)

    WB analysis of Jurkat using 14708-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PAPSS1 Polyclonal antibody (14708-1-AP)

    IP experiment of HepG2 using 14708-1-AP

    IP result of anti-PAPSS1 (IP:14708-1-AP, 4ug; Detection:14708-1-AP 1:1000) with HepG2 cells lysate 1000ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAPSS1 Polyclonal antibody (14708-1-AP)

    IHC staining of human ovary tumor using 14708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14708-1-AP (PAPSS1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAPSS1 Polyclonal antibody (14708-1-AP)

    IHC staining of human ovary tumor using 14708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14708-1-AP (PAPSS1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PAPSS1 Polyclonal antibody (14708-1-AP)

    IF Staining of HepG2 using 14708-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14708-1-AP (PAPSS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14708-1-AP targets PAPSS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAPSS1 fusion protein Ag6401
Full Name 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC050627
Gene Symbol PAPSS1
Gene ID (NCBI) 9061
RRIDAB_2159487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PAPSS1 antibody 14708-1-APDownload protocol
IHC protocol for PAPSS1 antibody 14708-1-APDownload protocol
IF protocol for PAPSS1 antibody 14708-1-APDownload protocol
IP protocol for PAPSS1 antibody 14708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,CoIP

Cancer Res

CIB2 negatively regulates oncogenic signaling in ovarian cancer via sphingosine kinase 1.

Authors - Wenying Zhu
View Article
hamsterWB

Biotechnol J

Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation.

Authors - Ren Liu
View Article
humanIHC

Histol Histopathol

Correlation of 3'-Phosphoadenosine-5'-Phosphosulfate synthase 1 (PAPSS1) expression with clinical parameters and prognosis in esophageal squamous carcinoma

Authors - Xuyou Zhu
View Article
humanWB

bioRxiv

Glycosaminoglycan-mediated lipoprotein uptake protects cancer cells from ferroptosis

Authors - Dylan Calhoon
View Article