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PAPOLB Polyclonal antibody

PAPOLB Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12821-1-AP

Synonyms

PAP beta, PAPOLB, PAPT, Poly(A) polymerase beta, TPAP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using PAPOLB Polyclonal antibody (12821-1-AP)

    WB analysis of mouse testis using 12821-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using PAPOLB Polyclonal antibody (12821-1-AP)

    WB analysis of human kidney using 12821-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PAPOLB Polyclonal antibody (12821-1-AP)

    WB analysis of HeLa using 12821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PAPOLB Polyclonal antibody (12821-1-AP)

    WB analysis of human brain using 12821-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using PAPOLB Polyclonal antibody (12821-1-AP)

    WB analysis of human stomach using 12821-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using PAPOLB Polyclonal antibody (12821-1-AP)

    WB analysis of mouse ovary using 12821-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using PAPOLB Polyclonal antibody (12821-1-AP)

    WB analysis of human placenta using 12821-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12821-1-AP (PAPOLB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse testis tissue, human kidney tissue, HeLa cells, human brain tissue, human stomach tissue, mouse ovary tissue, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12821-1-AP targets PAPOLB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAPOLB fusion protein Ag3487
Full Name poly(A) polymerase beta (testis specific)
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC036653
Gene Symbol PAPOLB
Gene ID (NCBI) 56903
RRIDAB_2159217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PAPOLB antibody 12821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols