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  • Featured Product
  • KD/KO Validated

PAPD5 Polyclonal antibody

PAPD5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 55197-1-AP

Synonyms

PAPD5, TENT4B, Terminal uridylyltransferase 3, TRF4 2, TUTase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PAPD5 Polyclonal antibody (55197-1-AP)

    WB analysis of HepG2 using 55197-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55197-1-AP (PAPD5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PAPD5 Polyclonal antibody (55197-1-AP)

    IP experiment of Jurkat using 55197-1-AP

    IP result of anti-PAPD5 (IP:55197-1-AP, 3ug; Detection:55197-1-AP 1:500) with Jurkat cells lysate 1600ug.

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55197-1-AP targets PAPD5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name PAP associated domain containing 5
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 63-80 kDa
GenBank Accession NumberNM_001040285
Gene Symbol PAPD5
Gene ID (NCBI) 64282
RRIDAB_2881287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAPD5, also named as Non-canonical poly(A) RNA polymerase PAPD5, is a 572 amino acid protein, which contains 1 PAP-associated domain and belongs to the DNA polymerase type-B-like family. PAPD5 is predominantly expressed in the cytoplasm. PAPD5 as catalytic subunit of a TRAMP-like complex that has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. PAPD5 plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. PAPD5 also may play a role in sister chromatid cohesion. The calculated molecular weight of PAPD5 is a 63 kDa, but the post-modified protein is about 70-80 kDa. PAPD5 exists some isoform with the molecular weight about 50 kDa and 70-80 kDa.

Protocols

Product Specific Protocols
WB protocol for PAPD5 antibody 55197-1-APDownload protocol
IP protocol for PAPD5 antibody 55197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

mRNAs are sorted for export or degradation before passing through nuclear speckles.

Authors - Jing Fan
  • KD Validated
View Article