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PANK1/2/3 Polyclonal antibody

PANK1/2/3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12466-1-AP

Synonyms

hPanK3, PANK1/2/3, PANK3, pantothenate kinase 3, Pantothenic acid kinase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    WB analysis of HepG2 using 12466-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12466-1-AP (PANK1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human hepatocirrhosis using 12466-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human hepatocirrhosis using 12466-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human liver cancer using 12466-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human liver cancer using 12466-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human liver cancer using 12466-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human liver cancer using 12466-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human breast cancer using 12466-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human breast cancer using 12466-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12466-1-AP targets PANK1/2/3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitysheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PANK1/2/3 fusion protein Ag3140
Full Name pantothenate kinase 3
Calculated Molecular Weight 370 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC013705
Gene Symbol PANK3
Gene ID (NCBI) 79646
RRIDAB_10644117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PANK1/2/3 antibody 12466-1-APDownload protocol
IHC protocol for PANK1/2/3 antibody 12466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepWB

Int J Mol Sci

MicroRNA-200c Affects Milk Fat Synthesis by Targeting PANK3 in Ovine Mammary Epithelial Cells

Authors - Zhiyun Hao
View Article