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  • Featured Product
  • KD/KO Validated

ORAI1 Polyclonal antibody

ORAI1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13130-1-AP

Synonyms

CRACM1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ORAI1 Polyclonal antibody (13130-1-AP)

    WB analysis of Jurkat using 13130-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ORAI1 Polyclonal antibody (13130-1-AP)

    WB analysis of Jurkat using 13130-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ORAI1 Polyclonal antibody (13130-1-AP)

    WB analysis of mouse spleen using 13130-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using ORAI1 Polyclonal antibody (13130-1-AP)

    WB analysis of mouse ovary using 13130-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using ORAI1 Polyclonal antibody (13130-1-AP)

    WB analysis of PC-3 using 13130-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using ORAI1 Polyclonal antibody (13130-1-AP)

    WB analysis of A375 using 13130-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ORAI1 Polyclonal antibody (13130-1-AP)

    IF Staining of HEK-293 using 13130-1-AP

    Immunofluorescent analysis of HEK-293 cells using 13130-1-AP (ORAI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, mouse ovary tissue, PC-3 cells, A375 cells, mouse spleen tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13130-1-AP targets ORAI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ORAI1 fusion protein Ag3758
Full Name ORAI calcium release-activated calcium modulator 1
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC015369
Gene Symbol ORAI1
Gene ID (NCBI) 84876
RRIDAB_2157558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Protocols

Product Specific Protocols
WB protocol for ORAI1 antibody 13130-1-APDownload protocol
IF protocol for ORAI1 antibody 13130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Res

Direct control of store-operated calcium channels by ultrafast laser.

Authors - Pan Cheng
  • KO Validated
View Article
mouseWB

Blood

Orai1 (CRACM1) is the platelet SOC channel and essential for pathological thrombus formation.

Authors - Braun Attila A
View Article
mouseWB

Blood

The serum- and glucocorticoid-inducible kinase 1 (SGK1) influences platelet calcium signaling and function by regulation of Orai1 expression in megakaryocytes.

Authors - Borst Oliver O
View Article
mouseWB

Kidney Int

AMP-activated kinase is a regulator of fibroblast growth factor 23 production.

Authors - Philipp Glosse
View Article
mouseIF

Sci Signal

Adhesion to laminin-1 and collagen IV induces the formation of Ca2+ microdomains that sensitize mouse T cells for activation

Authors - Mariella Weiß
View Article
mouseIF

Sci Signal

ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation.

Authors - Björn-Philipp Diercks
View Article