MCF7 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
MCF7 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10228-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
A431 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10228-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10228-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 10228-1-AP
IP result of anti-nucleobindin 1 (IP:10228-1-AP, 4ug; Detection:10228-1-AP 1:1000) with HepG2 cells lysate 2400ug.
IP result of anti-nucleobindin 1 (IP:10228-1-AP, 4ug; Detection:10228-1-AP 1:1000) with HepG2 cells lysate 2400ug.
IHC staining of human brain using 10228-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 10228-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 10228-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using nucleobindin 1 antibody (10228-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using nucleobindin 1 antibody (10228-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10228-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 10228-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug nucleobindin 1 Polyclonal antibody (10228-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug nucleobindin 1 Polyclonal antibody (10228-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Nucleobindin-1 (NUCB1), also known as Calnuc, is a highly conserved, multifunctional protein widely expressed in tissues and cells.It has been proposed to contain both calcium- and DNA-binding motifs, including two EF-hands, a basic amino-acid-rich region and a leucine zipper.Western blotting revealed a band at approximately 63 kDa, corresponding to the expected size of the NUCB1 protein(PMID: 15255074)
Protocols
Product Specific Protocols
WB protocol for Nucleobindin 1 antibody 10228-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10228-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10228-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10228-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human brain using 10228-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 10228-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HepG2 using 10228-1-AP
IP result of anti-nucleobindin 1 (IP:10228-1-AP, 4ug; Detection:10228-1-AP 1:1000) with HepG2 cells lysate 2400ug.
IF-P Figures
IF Staining of mouse brain using 10228-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using nucleobindin 1 antibody (10228-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 10228-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC (INTRA) Figures
FC experiment of HepG2 using 10228-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug nucleobindin 1 Polyclonal antibody (10228-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
Nucleobindin 1 Polyclonal antibody
Catalog Number
10228-1-AP
Citations
1
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
