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  • Featured Product
  • KD/KO Validated

NUAK2 Polyclonal antibody

NUAK2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11592-1-AP

Synonyms

NUAK family SNF1 like kinase 2, NUAK2, SNARK, SNF1/AMP kinase related kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using NUAK2 Polyclonal antibody (11592-1-AP)

    WB analysis of Y79 using 11592-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NUAK2 Polyclonal antibody (11592-1-AP)

    WB analysis of HeLa using 11592-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NUAK2 Polyclonal antibody (11592-1-AP)

    WB analysis of HeLa using 11592-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using NUAK2 Polyclonal antibody (11592-1-AP)

    WB analysis of Y79 using 11592-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using NUAK2 Polyclonal antibody (11592-1-AP)

    WB analysis of Y79 using 11592-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NUAK2 Polyclonal antibody (11592-1-AP)

    IHC staining of human kidney using 11592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NUAK2 Polyclonal antibody (11592-1-AP)

    IHC staining of human kidney using 11592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NUAK2 Polyclonal antibody (11592-1-AP)

    IHC staining of human renal cell carcinoma using 11592-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11592-1-AP (NUAK2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NUAK2 Polyclonal antibody (11592-1-AP)

    IHC staining of human renal cell carcinoma using 11592-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11592-1-AP (NUAK2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH3T3 cells using NUAK2 Polyclonal antibody (11592-1-AP)

    IF Staining of NIH3T3 cells using 11592-1-AP

    NIH3T3 cells on cover slips fixed and staining with anti-NUAK2 (11592-1-AP,1:100;Rabbit polyclonal -Red) and anti-pericentrin(centrosome Marker; McAb-Green). The photo shows the centrosome in cell cycle: Interphase, Prophase, Prometaphase, Metaphase, Anaphase and Telophase. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml.(from Dr. Zineldeen Doaa Hussein) .

Tested Applications

Positive WB detected inY79 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11592-1-AP targets NUAK2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUAK2 fusion protein Ag2031
Full Name NUAK family, SNF1-like kinase, 2
Calculated Molecular Weight 628 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC017306
Gene Symbol NUAK2
Gene ID (NCBI) 81788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUAK2(NUAK family SNF1-like kinase 2) is also named as OMPHK2, SNARK and belongs to the SNF1 subfamily. The function of NUAK2 is partly dependent on anti-apoptotic properties against apoptosis induced by death ligand and NUAK2 also has effects on the migration of cancer cells as speculated from studies on myosin filaments and cytoskeleton organization in normal cells(PMID:21911917). Western blot analysis detected NUAK2 expressed in cancer cell lines of diverse tissue origin(PMID:12409306).

Protocols

Product Specific Protocols
WB protocol for NUAK2 antibody 11592-1-APDownload protocol
IHC protocol for NUAK2 antibody 11592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Proc Natl Acad Sci U S A

AMP kinase-related kinase NUAK2 affects tumor growth, migration, and clinical outcome of human melanoma.

Authors - Namiki Takeshi T
View Article
humanWB, IF

Cancer Res

NUAK2 amplification coupled with PTEN deficiency promote melanoma development via CDK activation.

Authors - Takeshi Namiki
  • KD Validated
View Article
humanWB

Diabetologia

Regulation of skeletal muscle sucrose, non-fermenting 1/AMP-activated protein kinase-related kinase (SNARK) by metabolic stress and diabetes.

Authors - Rune A A
  • KD Validated
View Article
humanWB, IF

Ann Clin Biochem

Sucrose non-fermenting AMPK related kinase/Pentraxin 3 and DNA damage axis: a gateway to cardiovascular disease in systemic lupus erythematosus among Egyptian patients.

Authors - Doaa Hussein Zineldeen
View Article