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Neuropilin 1 Monoclonal antibody
Neuropilin 1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rabbit and More (2)
Applications
WB, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2H3F6
Cat no : 60067-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human placenta tissue, fetal human brain tissue, human plasma, 37°C incubated human placenta tissue, pig brain tissue, HeLa cells, rabbit heart tissue, MDA-MB-231 cells, A549 cells, pig heart tissue |
Positive IF/ICC detected in | SH-SY5Y cells, human embronic stem cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 11 publications below |
IF | See 10 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Product Information
60067-1-Ig targets Neuropilin 1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, pig, rabbit samples.
Tested Reactivity | human, pig, rabbit |
Cited Reactivity | human, mouse, rabbit, xenopus |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Neuropilin 1 fusion protein Ag0931 |
Full Name | neuropilin 1 |
Calculated Molecular Weight | 103 kDa |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | BC007737 |
Gene Symbol | Neuropilin 1 |
Gene ID (NCBI) | 8829 |
RRID | AB_2150840 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
Protocols
Product Specific Protocols | |
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WB protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
IF protocol for Neuropilin 1 antibody 60067-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Elife Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. | ||
Acta Biomater Semaphorin-3A, Neuropilin-1 and Plexin-A1 in Prosthetic-Particle Induced Bone Loss. | ||
J Pathol Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma. | ||
Am J Cancer Res CMTM6 promotes cell proliferation and invasion in oral squamous cell carcinoma by interacting with NRP1. | ||
Pharm Res Dual Receptor Targeting Cell Penetrating Peptide Modified Liposome for Glioma and Breast Cancer Postoperative Recurrence Therapy. | ||
Exp Eye Res Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury. |