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- KD/KO Validated
NRF1/nuclear respiratory factor 1 Polyclonal antibody
NRF1/nuclear respiratory factor 1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12482-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, human lung tissue, HEK-293T cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 80 publications below |
| IHC | See 1 publications below |
Product Information
12482-1-AP targets NRF1/nuclear respiratory factor 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, zebrafish, goat, ducks |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NRF1/nuclear respiratory factor 1 fusion protein Ag3172 |
| Full Name | nuclear respiratory factor 1 |
| Calculated Molecular Weight | 522 aa, 56 kDa |
| Observed Molecular Weight | 67 kDa, 45 kDa |
| GenBank Accession Number | BC016925 |
| Gene Symbol | NRF1 |
| Gene ID (NCBI) | 4899 |
| RRID | AB_2282876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-AP | Download protocol |
| IHC protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-AP | Download protocol |
| IP protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hazard Mater Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers | ||
Redox Biol Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy. | ||
Cell Commun Signal Local GHR roles in regulation of mitochondrial function through mitochondrial biogenesis during myoblast differentiation | ||
Mater Today Bio Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction |
