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NPBWR2 Polyclonal antibody

NPBWR2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 24162-1-AP

Synonyms

G protein coupled receptor 8, GPR8, neuropeptides B/W receptor 2, NPBWR2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using NPBWR2 Polyclonal antibody (24162-1-AP)

    IHC staining of human brain using 24162-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24162-1-AP (NPBWR2 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NPBWR2 Polyclonal antibody (24162-1-AP)

    IHC staining of human brain using 24162-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24162-1-AP (NPBWR2 Antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24162-1-AP is NPBWR2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPBWR2 fusion protein Ag20874
Full Name neuropeptides B/W receptor 2
Calculated Molecular Weight 333 aa, 37 kDa
GenBank Accession NumberBC067481
Gene Symbol NPBWR2
Gene ID (NCBI) 2832
RRIDAB_2879442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPBWR1 (GPR7) and NPBWR2 (GPR8) are two structurally related G-protein coupled receptors. These two receptors share high sequence identity to each other and significant similarity with opioid and somatostatin receptors (PMID: 10407191). Neuropeptide B (NPB) and neuropeptide W (NPW) are endogenous peptide ligands of these receptors. NPBWR1 was found highly conserved in both human and rodents while NPBWR2 gene was not found in the rodent genomes (PMID: 7590751; 10407191). NPBWR1 and NPBWR2 are implicated in energy homeostasis, pain, and emotion (PMID: 23515889).

Protocols

Product Specific Protocols
IHC protocol for NPBWR2 antibody 24162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols