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NPAS1 Polyclonal antibody

NPAS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13701-1-AP

Synonyms

bHLHe11, Member of PAS protein 5, MOP5, neuronal PAS domain protein 1, Neuronal PAS1, NPAS1, PASD5

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using NPAS1 Polyclonal antibody (13701-1-AP)

    WB analysis of human brain using 13701-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13701-1-AP (NPAS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using NPAS1 Polyclonal antibody (13701-1-AP)

    IHC staining of human brain using 13701-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13701-1-AP (NPAS1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NPAS1 Polyclonal antibody (13701-1-AP)

    IHC staining of human brain using 13701-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13701-1-AP (NPAS1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13701-1-AP targets NPAS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPAS1 fusion protein Ag4620
Full Name neuronal PAS domain protein 1
Calculated Molecular Weight 590 aa, 63 kDa
Observed Molecular Weight 63-68 kDa
GenBank Accession NumberBC039016
Gene Symbol NPAS1
Gene ID (NCBI) 4861
RRIDAB_10644122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NPAS1 antibody 13701-1-APDownload protocol
IHC protocol for NPAS1 antibody 13701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols