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NF-M-Specific Polyclonal antibody

NF-M-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 20664-1-AP

Synonyms

NEFM, NF-M, NF M, neurofilament, NEF3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    WB analysis of mouse brain using 20664-1-AP

    mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    WB analysis of mouse brain tissue using 20664-1-AP

    WB result of 20664-1-AP.

  • Western Blot (WB) analysis of mouse brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    WB analysis of mouse brain using 20664-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    WB analysis of human brain using 20664-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    WB analysis of human brain using 20664-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    WB analysis of rat brain using 20664-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of rat brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    IP experiment of rat brain using 20664-1-AP

    IP result of anti-NF-M-Specific (IP:20664-1-AP, 4ug; Detection:20664-1-AP 1:1000) with rat brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    IHC staining of human brain using 20664-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    IHC staining of human brain using 20664-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    IHC staining of rat brain using 20664-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of rat brain tissue using NF-M-Specific Polyclonal antibody (20664-1-AP)

    IHC staining of rat brain using 20664-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using NF-M-Specific Polyclonal antibody (20664-1-AP)

    IF Staining of SH-SY5Y using 20664-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20664-1-AP (NF-M-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of PC-12 cells using NF-M-Specific Polyclonal antibody (20664-1-AP)

    FC experiment of PC-12 using 20664-1-AP

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M-Specific (20664-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, human brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20664-1-AP targets NF-M-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name neurofilament, medium polypeptide
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 140-160 kDa
GenBank Accession NumberNM_005382
Gene Symbol NF-M
Gene ID (NCBI) 4741
RRIDAB_10700009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. The antibody is specific to NEFM.

Protocols

Product Specific Protocols
WB protocol for NF-M-Specific antibody 20664-1-APDownload protocol
IHC protocol for NF-M-Specific antibody 20664-1-APDownload protocol
IF protocol for NF-M-Specific antibody 20664-1-APDownload protocol
IP protocol for NF-M-Specific antibody 20664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Neurofilament Light Regulates Axon Caliber, Synaptic Activity, and Organelle Trafficking in Cultured Human Motor Neurons.

Authors - Markus T Sainio
View Article
mouseWB

Hum Mol Genet

Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Authors - Peng-Peng Zhu
View Article