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NECAP1 Polyclonal antibody

NECAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17906-1-AP

Synonyms

NECAP 1, NECAP endocytosis associated 1, NECAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NECAP1 Polyclonal antibody (17906-1-AP)

    WB analysis using 17906-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17906-1-AP (NECAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NECAP1 Polyclonal antibody (17906-1-AP)

    WB analysis of human brain using 17906-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17906-1-AP (NECAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NECAP1 Polyclonal antibody (17906-1-AP)

    IP experiment of mouse brain using 17906-1-AP

    IP result of anti-NECAP1 (IP:17906-1-AP, 4ug; Detection:17906-1-AP 1:300) with mouse brain tissue lysate 6000 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NECAP1 Polyclonal antibody (17906-1-AP)

    IHC staining of human kidney using 17906-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17906-1-AP (NECAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NECAP1 Polyclonal antibody (17906-1-AP)

    IHC staining of human kidney using 17906-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17906-1-AP (NECAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

17906-1-AP targets NECAP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NECAP1 fusion protein Ag12283
Full Name NECAP endocytosis associated 1
Calculated Molecular Weight 275 aa, 30 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC110876
Gene Symbol NECAP1
Gene ID (NCBI) 25977
RRIDAB_2149177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NECAP1 antibody 17906-1-APDownload protocol
IHC protocol for NECAP1 antibody 17906-1-APDownload protocol
IP protocol for NECAP1 antibody 17906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Med Genet

NECAP1 loss of function leads to a severe infantile epileptic encephalopathy.

Authors - Anas M Alazami
View Article
mouse

Sci Rep

Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.

Authors - Ermes Candiello
View Article