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NDUFA3 Polyclonal antibody

NDUFA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17257-1-AP

Synonyms

NADH-ubiquinone oxidoreductase B9 subunit, Complex I-B9, CI-B9, CIB9, CI B9

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using NDUFA3 Polyclonal antibody (17257-1-AP)

    WB analysis of human brain using 17257-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NDUFA3 Polyclonal antibody (17257-1-AP)

    WB analysis of HeLa using 17257-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFA3 Polyclonal antibody (17257-1-AP)

    IHC staining of human liver using 17257-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using NDUFA3 Polyclonal antibody (17257-1-AP)

    IHC staining of human stomach using 17257-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFA3 Polyclonal antibody (17257-1-AP)

    IHC staining of human liver using 17257-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17257-1-AP (NDUFA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

17257-1-AP targets NDUFA3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFA3 fusion protein Ag10912
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa
Calculated Molecular Weight65aa,7 kDa; 84aa,9 kDa
Observed Molecular Weight 9 kDa
GenBank Accession NumberBC011021
Gene Symbol NDUFA3
Gene ID (NCBI) 4696
RRIDAB_2150631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDUFA3 antibody 17257-1-APDownload protocol
IHC protocol for NDUFA3 antibody 17257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong
View Article
humanWB

J Biol Chem

Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.

Authors - Yanchun Ji
View Article
humanWB

Invest Ophthalmol Vis Sci

Assocation Between Leber's Hereditary Optic Neuropathy and MT-ND1 3460G>A Mutation-Induced Alterations in Mitochondrial Function, Apoptosis, and Mitophagy.

Authors - Juanjuan Zhang
View Article