NCOR1 Polyclonal antibody
NCOR1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 20018-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells |
| Positive IHC detected in | human cervical cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
20018-1-AP targets NCOR1 in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | nuclear receptor co-repressor 1 |
| Calculated Molecular Weight | 270 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | NM_006311 |
| Gene Symbol | NCOR1 |
| Gene ID (NCBI) | 9611 |
| RRID | AB_10665360 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NCOR1, also named as KIAA1047 and TRAC1, belongs to the N-CoR nuclear receptor corepressors family. NCO1 mediates transcriptional repression by certain nuclear receptors. It is part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. The antibody recognizes the 103 kDa isoform of NCOR1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NCOR1 antibody 20018-1-AP | Download protocol |
| IHC protocol for NCOR1 antibody 20018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease | ||
PLoS Biol Transcriptional programming of lipid and amino acid metabolism by the skeletal muscle circadian clock. | ||
iScience WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation | ||
Theranostics Palmitoylation of PKCδ by ZDHHC5 in hypothalamic microglia presents as a therapeutic target for fatty liver disease | ||
Cell Osr2 functions as a biomechanical checkpoint to aggravate CD8+ T cell exhaustion in tumor | ||
Cell Mol Life Sci Novel role of NCoR1 in impairing spatial memory through the mediation of a novel interacting protein DEC2
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