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MCL1L-specific Polyclonal antibody

MCL1L-specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 15825-1-AP

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human spleen tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human spleen using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human spleen using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human kidney using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human kidney using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human placenta using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human placenta using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human testis using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human testis using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human skin using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human skin using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human brain using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human brain using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human spleen using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human spleen using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human lung using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human lung using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human ovary using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human ovary using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15825-1-AP (MCL1L-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human breast cancer using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human breast cancer using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human cervical cancer using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human cervical cancer using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human tonsillitis using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MCL1L-specific Polyclonal antibody (15825-1-AP)

    IHC staining of human tonsillitis using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman spleen tissue, human brain tissue, human breast cancer tissue, human cervical cancer tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15825-1-AP targets MCL1L-specific in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Calculated Molecular Weight 37 kDa
GenBank Accession NumberNM_182763
Gene Symbol MCL1
Gene ID (NCBI) 4170
RRIDAB_2144112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. This antibody is specific to MCL1L isoform.

Protocols

Product Specific Protocols
IHC protocol for MCL1L-specific antibody 15825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols