- Featured Product
- KD/KO Validated
Marcksl1 Polyclonal antibody
Marcksl1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10002-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
Product Information
10002-2-AP targets Marcksl1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein |
| Full Name | MARCKS-like 1 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC006757 |
| Gene Symbol | Marcksl1 |
| Gene ID (NCBI) | 17357 |
| RRID | AB_513892 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MacMARCKS is a member of MARCKS family of PKC substrate. It is widely used in the signal transduction studies as an indictor of PKC activation. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-45 kDa in different species on SDS PAGE. MacMARCKS functions in many aspects of cell physiology including integrin activation, cell spreading and phagocytosis. Its functions are though to associate with its ability in binding and regulating actin and microtubule cytoskeleton. Current evidence indicated that this protein also involved controls membrane fluidity and molecular mobility of membrane proteins
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Marcksl1 antibody 10002-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling. | ||
Dev Cell An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration | ||
J Biol Chem The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.
| ||
Mol Cell Biol c-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells.
| ||
PLoS One Colonic miRNA Expression/Secretion, Regulated by Intestinal Epithelial PepT1, Plays an Important Role in Cell-to-Cell Communication during Colitis. | ||
