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MYO16 Polyclonal antibody

MYO16 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18828-1-AP

Synonyms

NYAP3, Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 3, MYR8, myosin XVI, MYO16B

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human bladder tissue using MYO16 Polyclonal antibody (18828-1-AP)

    IHC staining of human bladder using 18828-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using MYO16 Polyclonal antibody (18828-1-AP)

    IHC staining of human bladder using 18828-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MYO16 Polyclonal antibody (18828-1-AP)

    IHC staining of human testis using 18828-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MYO16 Polyclonal antibody (18828-1-AP)

    IHC staining of human testis using 18828-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using MYO16 Polyclonal antibody (18828-1-AP)

    IHC staining of human bladder using 18828-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using MYO16 Polyclonal antibody (18828-1-AP)

    IHC staining of human bladder using 18828-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MYO16 Polyclonal antibody (18828-1-AP)

    IF Staining of A431 using 18828-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 18828-1-AP (MYO16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman bladder tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18828-1-AP targets MYO16 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin XVI
Calculated Molecular Weight 1858 aa, 206 kDa
Observed Molecular Weight 123 kDa, 210 kDa, 106 kDa
GenBank Accession NumberBC146791
Gene Symbol MYO16
Gene ID (NCBI) 23026
RRIDAB_10858634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO16, also named as KIAA0865, MYO16B, is actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO16 may be involved in targeting of the catalytic subunit of protein phosphatase 1 during brain development. The antibody can recognize isoform1, isoform2 and isoform3 of MYO16.

Protocols

Product Specific Protocols
IHC protocol for MYO16 antibody 18828-1-APDownload protocol
IF protocol for MYO16 antibody 18828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols