MYC-tag Monoclonal antibody

MYC-tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 60003-1-Ig

Synonyms



Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60003-1-Ig targets MYC-tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name MYC-tag
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Publications

SpeciesApplicationTitle
WB

Nat Commun

Metabolic regulation of telomere silencing by SESAME complex-catalyzed H3T11 phosphorylation.

Authors - Shihao Zhang
IP

Cell Death Differ

Bcl-3 promotes TNF-induced hepatocyte apoptosis by regulating the deubiquitination of RIP1

Authors - Yiming Hu
WB, IF

Autophagy

Chaperone-mediated autophagy controls the turnover of E3 ubiquitin ligase MARCHF5 and regulates mitochondrial dynamics.

Authors - Tiejian Nie
WB

Plant Cell

Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.

Authors - Cuicui Shen
IP

Proc Natl Acad Sci U S A

Inactivation of the tumor suppressor p53 by long noncoding RNA RMRP.

Authors - Yajie Chen

J Mol Cell Biol

BCL7C suppresses ovarian cancer growth by inactivating mutant p53.

Authors - Canhua Huang