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MUM1 Polyclonal antibody

MUM1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12682-1-AP

Synonyms

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere, PWWP3A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MUM1 Polyclonal antibody (12682-1-AP)

    WB analysis using 12682-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MUM1 Polyclonal antibody (12682-1-AP)

    WB analysis of Jurkat using 12682-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using MUM1 Polyclonal antibody (12682-1-AP)

    WB analysis of HT-1080 using 12682-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using MUM1 Polyclonal antibody (12682-1-AP)

    WB analysis of HT-1080 using 12682-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using MUM1 Polyclonal antibody (12682-1-AP)

    IP experiment of Jurkat using 12682-1-AP

    IP result of anti-MUM1 (IP:12682-1-AP, 4ug; Detection:12682-1-AP 1:300) with Jurkat cells lysate 4000ug.

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HT-1080 cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12682-1-AP targets MUM1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUM1 fusion protein Ag3371
Full Name melanoma associated antigen (mutated) 1
Calculated Molecular Weight 18 kDa, 79 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC008098
Gene Symbol MUM1
Gene ID (NCBI) 84939
RRIDAB_10642694
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.

Protocols

Product Specific Protocols
WB protocol for MUM1 antibody 12682-1-APDownload protocol
IP protocol for MUM1 antibody 12682-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Post-translational control of beige fat biogenesis by PRDM16 stabilization

Authors - Qiang Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Aurelie (Verified Customer) (09-01-2019)

Lysats (20µg) from Mouse cortex were loaded in a 8% SDS-PAGE and immunoblotted using anti-MUM1 antibody ( 1/1000) over-night at 4°C.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse Cortex
MUM1 Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex (Cat no:12682-1-AP)