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  • Featured Product
  • KD/KO Validated

AEG-1/MTDH Polyclonal antibody

AEG-1/MTDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 13860-1-AP

Synonyms

AEG-1/MTDH-Specific, AEG-1, MTDH, Astrocyte elevated gene-1 protein, AEG1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of MCF-7 using 13860-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of HeLa using 13860-1-AP

    WB result of AEG-1/MTDH-Specific antibody (13860-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AEG-1/MTDH-Specific transfected HeLa cells.

  • Western Blot (WB) analysis of A549 cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of A549 cells using 13860-1-AP

    WB result of AEG-1/MTDH antibody (13860-1-AP, 1:2000) with si-Control and si-MTDH transfected A549 cells.

  • Western Blot (WB) analysis of RAW 264.7 cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of RAW 264.7 using 13860-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of A375 using 13860-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis using 13860-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of mouse spleen using 13860-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of HeLa using 13860-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of A549 using 13860-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of DU 145 using 13860-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of PC-3 using 13860-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    WB analysis of HepG2 using 13860-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    IP experiment of MCF-7 using 13860-1-AP

    IP result of anti-AEG-1/MTDH-Specific (IP:13860-1-AP, 3ug; Detection:13860-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    IHC staining of human breast cancer using 13860-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    IHC staining of human breast cancer using 13860-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    IHC staining of human liver cancer using 13860-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    IHC staining of mouse brain using 13860-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    IHC staining of mouse brain using 13860-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AEG-1/MTDH-Specific Polyclonal antibody (13860-1-AP)

    IHC staining of human liver cancer using 13860-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AEG-1/MTDH Polyclonal antibody (13860-1-AP)

    IF Staining of MCF-7 using 13860-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AEG-1/MTDH-Specific antibody (13860-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inMCF-7 cells, A375 cells, HeLa cells, DU 145 cells, HL-60 cells, A549 cells, mouse spleen tissue, PC-3 cells, HepG2 cells, RAW 264.7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13860-1-AP targets AEG-1/MTDH in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AEG-1/MTDH fusion protein Ag4840
Full Name metadherin
Calculated Molecular Weight 582 aa, 64 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC045642
Gene Symbol AEG1/MTDH
Gene ID (NCBI) 92140
RRIDAB_2266734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for AEG-1/MTDH antibody 13860-1-APDownload protocol
IHC protocol for AEG-1/MTDH antibody 13860-1-APDownload protocol
IF protocol for AEG-1/MTDH antibody 13860-1-APDownload protocol
IP protocol for AEG-1/MTDH antibody 13860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF, WB, IP, IHC

J Crohns Colitis

MTDH promotes intestinal inflammation by positively regulating TLR signaling.

Authors - Lijuan Wang
View Article
humanWB

Oncogene

MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo.

Authors - He X-X XX
View Article

Mol Ther

Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells.

Authors - Hao Teng
View Article
humanWB

Mol Ther Nucleic Acids

PMID: 28624193

Authors - Yin-Ping Fan
View Article
humanWB,IHC

Oncotarget

MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1.

Authors - Jing-Jun Yan
View Article
humanIHC

Int J Cancer

Increased expression of metadherin protein predicts worse disease-free and overall survival in laryngeal squamous cell carcinoma.

Authors - Liu Yong Y
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

eman (Verified Customer) (11-26-2020)

this antibody works very well incubating either at room temperature or overnight2 dilutions are tested 1:1000 and 1:2000IF not tested yet

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000 and 1:2000
  • Cell Tissue Type: Hela cells
AEG-1/MTDH Antibody Western Blot,Immunoprecipitation validation (1:1000 and 1:2000 dilution) in Hela cells (Cat no:13860-1-AP)