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MRPS9 Polyclonal antibody

MRPS9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16533-1-AP

Synonyms

Small ribosomal subunit protein uS9m, S9mt, RPMS9, MRP-S9, MRP S9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS9 Polyclonal antibody (16533-1-AP)

    WB analysis using 16533-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16533-1-AP (MRPS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MRPS9 Polyclonal antibody (16533-1-AP)

    WB analysis of HEK-293 using 16533-1-AP

    WB result of MRPS9 antibody (16533-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS9 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using MRPS9 Polyclonal antibody (16533-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 16533-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16533-1-AP (MRPS9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16533-1-AP targets MRPS9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS9 fusion protein Ag9809
Full Name mitochondrial ribosomal protein S9
Calculated Molecular Weight 396 aa, 46 kDa
Observed Molecular Weight 38-45 kDa
GenBank Accession NumberBC057240
Gene Symbol MRPS9
Gene ID (NCBI) 64965
RRIDAB_2878272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPS9 antibody 16533-1-APDownload protocol
IHC protocol for MRPS9 antibody 16533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

C17orf80 binds the mitochondrial genome to promote its replication

Authors - Hao Wu
View Article