MRPS27 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, IP|
|Cited applications||IF, WB|
|Species specificity||Human,Mouse,Rat; other species not tested.|
|Cited species||human, mouse|
|Positive WB detected in||HeLa cells, A431 cells, human heart tissue, human skeletal muscle tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human kidney tissue, human lung tissue, human spleen tissue, human testis tissue|
|Positive IF detected in||HepG2 cells|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- HeLa cells were subjected to SDS PAGE followed by western blot with 17280-1-AP(MRPS27 antibody) at dilution of 1:500
- Immunohistochemical of paraffin-embedded human kidney using 17280-1-AP(MRPS27 antibody) at dilution of 1:100 (under 40x lens)
- Immunofluorescent analysis of HepG2 cells, using MRPS27 antibody 17280-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- IP Result of anti-MRPS27 (IP:17280-1-AP, 3ug; Detection:17280-1-AP 1:1000) with A431 cells lysate 1520ug.
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||MRPS27 fusion protein ag11047||Full name||mitochondrial ribosomal protein S27|
|168aa,19 kDa,48 kDa||
|BC030521||Gene ID (NCBI)||23107|
|Gene symbol||MRPS27||Synonyms||FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt|
Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].