MRPS15 Polyclonal antibody

Name: MRPS15 Polyclonal antibody
Brand: Proteintech
SKU: 17006-1-AP

MRPS15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17006-1-AP

Synonyms

DC37, MPR S15, MRP S15, MRPS15, RPMS15, S15mt

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis using 17006-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17006-1-AP (MRPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 17006-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17006-1-AP (MRPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 17006-1-AP
IP result of anti-MRPS15 (IP:17006-1-AP, 4ug; Detection:17006-1-AP 1:300) with mouse brain tissue lysate 3200 ug.
IHC staining of human spleen using 17006-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17006-1-AP (MRPS15 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17006-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17006-1-AP (MRPS15 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 17006-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPS15 rabbit poly (17006-1-AP, Green) and KRT18 mouse mAb (66187-1-Ig, Red) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L) for 17006-1-AP, and Alexa Fluor 594-conjugated Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

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Cat No. 17006-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, mouse liver tissue, MCF-7 cells, Raji cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 12 publications below

Product Information

17006-1-AP targets MRPS15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MRPS15 fusion protein Ag10702
Full Name	mitochondrial ribosomal protein S15
Calculated Molecular Weight	257 aa, 30 kDa
Observed Molecular Weight	25-30 kDa
GenBank Accession Number	BC031336
Gene Symbol	MRPS15
Gene ID (NCBI)	64960
RRID	AB_2301068
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPS15, also named as RPMS15 or DC37, is a 257 amino acid protein, which belongs to the ribosomal protein S15P family. MRPS15 localizes in the Mitochondrion and is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. MRPS15 is involved into the mitochondrial proteins biosynthesis and ribosomal biogenesis.

Protocols

Product Specific Protocols
WB protocol for MRPS15 antibody 17006-1-AP	Download protocol
IHC protocol for MRPS15 antibody 17006-1-AP	Download protocol
IF protocol for MRPS15 antibody 17006-1-AP	Download protocol
IP protocol for MRPS15 antibody 17006-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell An in vitro system to silence mitochondrial gene expression. Authors - Luis Daniel Cruz-Zaragoza View Article
human	WB	Cell Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein. Authors - Ricarda Richter-Dennerlein View Article
human	WB	Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria Authors - Sabine Poerschke View Article
human	WB	Cell Metab Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids. Authors - Daniel F Bogenhagen View Article
human	WB	Nucleic Acids Res The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis. Authors - Elena Lavdovskaia View Article
human	WB	Hum Mol Genet Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement. Authors - Juliette Pulman View Article

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MRPS15 Polyclonal antibody

MRPS15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 17006-1-AP

WB analysis using 17006-1-AP

IP experiment of mouse brain using 17006-1-AP

IHC staining of human spleen using 17006-1-AP

IHC staining of human spleen using 17006-1-AP

IF Staining of HepG2 using 17006-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An in vitro system to silence mitochondrial gene expression.

Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.