• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MRPS14 Polyclonal antibody

MRPS14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16301-1-AP

Synonyms

HSMRPS14, MRP S14, MRPS14, S14mt

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using MRPS14 Polyclonal antibody (16301-1-AP)

    WB analysis of mouse liver using 16301-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16301-1-AP (MRPS14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MRPS14 Polyclonal antibody (16301-1-AP)

    IHC staining of mouse liver using 16301-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16301-1-AP (MRPS14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MRPS14 Polyclonal antibody (16301-1-AP)

    IHC staining of mouse liver using 16301-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16301-1-AP (MRPS14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

16301-1-AP targets MRPS14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS14 fusion protein Ag9018
Full Name mitochondrial ribosomal protein S14
Calculated Molecular Weight 128 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC009788
Gene Symbol MRPS14
Gene ID (NCBI) 63931
RRIDAB_2878240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPS14 antibody 16301-1-APDownload protocol
IHC protocol for MRPS14 antibody 16301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
View Article
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
View Article
humanWB

Nat Commun

Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.

Authors - Hauke S Hillen
View Article
humanWB

Nucleic Acids Res

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Authors - Elena Lavdovskaia
View Article
humanWB

Elife

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.

Authors - Sven Dennerlein
View Article