Filter: AllWBIHCIF/ICC

WB analysis of mouse brain using 13677-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13677-1-AP (MPPED1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 13677-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 13677-1-AP (MPPED1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 13677-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 13677-1-AP (MPPED1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 13677-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 13677-1-AP (MPPED1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 13677-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13677-1-AP (MPPED1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 13677-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13677-1-AP (MPPED1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 13677-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13677-1-AP (MPPED1 Antibody) at dilution of 1:50(under 10x lens).
IHC staining of human heart using 13677-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13677-1-AP (MPPED1 Antibody) at dilution of 1:50(under 40x lens).
IF Staining of HeLa using 13677-1-AP
Immunofluorescent analysis of Hela cells, using MPPED1 antibody 13677-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

MPPED1 Polyclonal antibody

Name: MPPED1 Polyclonal antibody
Brand: Proteintech
SKU: 13677-1-AP

MPPED1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13677-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Metallophosphoesterase domain-containing protein 1, FAM1A, EC:3.1.-.-, C22orf1, 239AB

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IHC detected in	human pancreas tissue, human brain tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13677-1-AP targets MPPED1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MPPED1 fusion protein Ag4672 Predict reactive species
Full Name	metallophosphoesterase domain containing 1
Calculated Molecular Weight	326 aa, 37 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC028035
Gene Symbol	MPPED1
Gene ID (NCBI)	758
RRID	AB_10644336
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15442
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MPPED1 antibody 13677-1-AP	Download protocol
IHC protocol for MPPED1 antibody 13677-1-AP	Download protocol
IF protocol for MPPED1 antibody 13677-1-AP	Download protocol