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MOSC2 Polyclonal antibody

MOSC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24782-1-AP

Synonyms

MOSC domain-containing protein 2, Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MOSC2 Polyclonal antibody (24782-1-AP)

    WB analysis using 24782-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24782-1-AP (MOSC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MOSC2 Polyclonal antibody (24782-1-AP)

    WB analysis of mouse liver using 24782-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24782-1-AP (MOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MOSC2 Polyclonal antibody (24782-1-AP)

    IHC staining of mouse liver using 24782-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24782-1-AP (MOSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MOSC2 Polyclonal antibody (24782-1-AP)

    IHC staining of mouse liver using 24782-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24782-1-AP (MOSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MOSC2 Polyclonal antibody (24782-1-AP)

    IHC staining of human liver cancer using 24782-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24782-1-AP (MOSC2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MOSC2 Polyclonal antibody (24782-1-AP)

    IHC staining of human liver cancer using 24782-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24782-1-AP (MOSC2 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24782-1-AP targets MOSC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOSC2 fusion protein Ag20694
Full Name MOCO sulphurase C-terminal domain containing 2
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC011973
Gene Symbol MOSC2
Gene ID (NCBI) 54996
RRIDAB_2879719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOSC domain-containing protein 2 (also known as MOSC2), also known as MARC2, is a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. Also, MOSC2 may be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenou1s nitric oxide levels and biosynthesis. The reductase activity is regulated under adipogenic conditions, and down-regulation of the terminal component MOSC2 resulted in decreased lipid synthesis, suggesting a possible physiological role of this enzyme system and its component MOSC2 in lipogenesis(PMID: 22203676).

Protocols

Product Specific Protocols
WB protocol for MOSC2 antibody 24782-1-APDownload protocol
IHC protocol for MOSC2 antibody 24782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

RNA Sequencing of Collecting Duct Renal Cell Carcinoma Suggests an Interaction between miRNA and Target Genes and a Predominance of Deregulated Solute Carrier Genes.

Authors - Sven Wach
View Article