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MOBKL3 Polyclonal antibody

MOBKL3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15886-1-AP

Synonyms

PHOCN, Mps one binder kinase activator-like 3, MOB4, CGI-95, 2C4D

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using MOBKL3 Polyclonal antibody (15886-1-AP)

    WB analysis of K-562 using 15886-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15886-1-AP (MOBKL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using MOBKL3 Polyclonal antibody (15886-1-AP)

    IP experiment of K-562 using 15886-1-AP

    IP result of anti-MOBKL3 (IP:15886-1-AP, 4ug; Detection:15886-1-AP 1:300) with K-562 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MOBKL3 Polyclonal antibody (15886-1-AP)

    IHC staining of human colon cancer using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MOBKL3 Polyclonal antibody (15886-1-AP)

    IHC staining of human colon cancer using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using MOBKL3 Polyclonal antibody (15886-1-AP)

    IHC staining of human stomach using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15886-1-AP (MOBKL3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using MOBKL3 Polyclonal antibody (15886-1-AP)

    IHC staining of human stomach using 15886-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15886-1-AP (MOBKL3 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15886-1-AP targets MOBKL3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOBKL3 fusion protein Ag8671
Full Name MOB1, Mps One Binder kinase activator-like 3 (yeast)
Calculated Molecular Weight 225 aa, 26 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC005237
Gene Symbol MOBKL3
Gene ID (NCBI) 25843
RRIDAB_10858478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MOBKL3 antibody 15886-1-APDownload protocol
IHC protocol for MOBKL3 antibody 15886-1-APDownload protocol
IP protocol for MOBKL3 antibody 15886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols