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MME,CD10 Monoclonal antibody
MME,CD10 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4B9D9
Cat no : 60034-3-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig kidney tissue, mouse kidney tissue, HEK-293 cells |
Positive IHC detected in | human kidney tissue, human liver tissue, human renal cell carcinoma tissue, human stomach cancer tissue, human tonsil tissue, mouse kidney tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:25000-1:100000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
60034-3-Ig targets MME,CD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Reactivity | human, mouse, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MME,CD10 fusion protein Ag0427 |
Full Name | membrane metallo-endopeptidase |
Calculated Molecular Weight | 750 aa, 86 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC101658 |
Gene Symbol | CD10 |
Gene ID (NCBI) | 4311 |
RRID | AB_10641991 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).
Protocols
Product Specific Protocols | |
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WB protocol for MME,CD10 antibody 60034-3-Ig | Download protocol |
IHC protocol for MME,CD10 antibody 60034-3-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |