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MNK1 Polyclonal antibody

MNK1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10136-1-AP

Synonyms

MKNK1, MAPK signal-integrating kinase 1, MAP kinase-interacting serine/threonine-protein kinase 1, MAP kinase signal-integrating kinase 1, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of HeLa using 10136-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of mouse small intestine using 10136-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of HL-60 using 10136-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of HEK-293 using 10136-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MNK1 Polyclonal antibody (10136-1-AP)

    WB analysis of HepG2 using 10136-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MNK1 Polyclonal antibody (10136-1-AP)

    IF Staining of HepG2 using 10136-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MNK1 antibody (10136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, HL-60 cells, mouse small intestine tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

10136-1-AP targets MNK1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MNK1 fusion protein Ag0184
Full Name MAP kinase interacting serine/threonine kinase 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa, 47 kDa
GenBank Accession NumberBC002755
Gene Symbol MNK1
Gene ID (NCBI) 8569
RRIDAB_2250527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MNK1 (mitogen-activated protein kinase-interacting kinase 1) was discovered as a consequence of bacterial expression libraries screening for proteins regulated by the classical mitogen-activated protein kinases, the ERKs (extracellular signal-regulated kinases). Phosphorylation of Mnk1 has been shown to activate its kinase activity as well as to enhance its binding to the eukaryotic initiation factor 4G (eIF4G) which functions as a scaffolding protein. Human MNK1 arises from an alternatively spliced transcript giving rise to 2 isoforms, longer MNK1a (50 kDa) and a shorter MNK1b variant (38 kDa) that lacks 89 C-terminal amino acids(PMID: 21406405).

Protocols

Product Specific Protocols
WB protocol for MNK1 antibody 10136-1-APDownload protocol
IF protocol for MNK1 antibody 10136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Novel HSP90-PI3K Dual Inhibitor Suppresses Melanoma Cell Proliferation by Interfering with HSP90-EGFR Interaction and Downstream Signaling Pathways.

Authors - Qian Zhao
View Article
humanWB

J Enzyme Inhib Med Chem

Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.

Authors - Feifei Qin
View Article
mouseWB

Chem Biol Drug Des

Caffeic acid-grafted chitooligosaccharides downregulate MAPK and NF-kB in RAW264.7 cells

Authors - Van-Hoai Bui
View Article