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METTL7B Polyclonal antibody

METTL7B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17001-1-AP

Synonyms

methyltransferase like 7B, METTL7B

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using METTL7B Polyclonal antibody (17001-1-AP)

    WB analysis using 17001-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17001-1-AP (METTL7B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using METTL7B Polyclonal antibody (17001-1-AP)

    IHC staining of mouse liver using 17001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17001-1-AP (METTL7B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17001-1-AP targets METTL7B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL7B fusion protein Ag10694
Full Name methyltransferase like 7B
Calculated Molecular Weight244aa,28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC020509
Gene Symbol METTL7B
Gene ID (NCBI) 196410
RRIDAB_2142199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for METTL7B antibody 17001-1-APDownload protocol
IHC protocol for METTL7B antibody 17001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome.

Authors - Daniel Fox
View Article
humanIHC

Front Mol Biosci

Characterization of METTL7B to Evaluate TME and Predict Prognosis by Integrative Analysis of Multi-Omics Data in Glioma

Authors - Xiaochuan Chen
View Article
humanWB

Ann Transl Med

METTL7B (methyltransferase-like 7B) identification as a novel biomarker for lung adenocarcinoma.

Authors - Jawad Ali
View Article
human,mouseWB

J Oncol

Downregulation of hsa-miR-30b-3p Inhibits the Oncogenicity of Lung Adenocarcinoma by Targeting the METTL7B Gene.

Authors - Ning Li
View Article
mouseWB

iScience

TWIK-related acid-sensitive K+ channel 2 promotes renal fibrosis by inducing cell-cycle arrest

Authors - Jian Zhang
View Article
humanWB

Front Immunol

Heart failure-related genes associated with oxidative stress and the immune landscape in lung cancer

Authors - Ruoshu Duan
View Article