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MEP1A Polyclonal antibody

MEP1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24640-1-AP

Synonyms

PPHA, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, Meprin A subunit alpha, Endopeptidase-2, EC:3.4.24.18

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MEP1A Polyclonal antibody (24640-1-AP)

    WB analysis using 24640-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24640-1-AP (MEP1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using MEP1A Polyclonal antibody (24640-1-AP)

    WB analysis of mouse kidney using 24640-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24640-1-AP (MEP1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MEP1A Polyclonal antibody (24640-1-AP)

    IHC staining of human colon cancer using 24640-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 24640-1-AP (MEP1A antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

24640-1-AP targets MEP1A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEP1A fusion protein Ag18950
Full Name meprin A, alpha (PABA peptide hydrolase)
Calculated Molecular Weight 746 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC131714
Gene Symbol MEP1A
Gene ID (NCBI) 4224
RRIDAB_2879650
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MEP1A antibody 24640-1-APDownload protocol
IHC protocol for MEP1A antibody 24640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Comput Struct Biotechnol J

Integrated immunogenomic analysis of single-cell and bulk profiling reveals novel tumor antigens and subtype-specific therapeutic agents in lung adenocarcinoma

Authors - Saisai Tian
View Article