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MELK Polyclonal antibody

MELK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11403-1-AP

Synonyms

hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using MELK Polyclonal antibody (11403-1-AP)

    WB analysis of Jurkat using 11403-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MELK Polyclonal antibody (11403-1-AP)

    WB analysis using 11403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MELK Polyclonal antibody (11403-1-AP)

    WB analysis of K-562 using 11403-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of human breast cancer using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of human breast cancer using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of human colon cancer using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of mouse colon using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of mouse colon using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of mouse colon using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of mouse kidney using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of mouse kidney using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of human lung cancer using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of human lung cancer using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MELK Polyclonal antibody (11403-1-AP)

    IHC staining of human colon cancer using 11403-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, mouse colon tissue, mouse kidney tissue, human lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11403-1-AP targets MELK in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MELK fusion protein Ag1897
Full Name maternal embryonic leucine zipper kinase
Calculated Molecular Weight 651 aa, 75 kDa
Observed Molecular Weight 50-70 kDa
GenBank Accession NumberBC014039
Gene Symbol MELK
Gene ID (NCBI) 9833
RRIDAB_2877762
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

Protocols

Product Specific Protocols
WB protocol for MELK antibody 11403-1-APDownload protocol
IHC protocol for MELK antibody 11403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,CoIP

Int J Oncol

MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway.

Authors - Jiecheng Ye
View Article
humanIHC

Med Sci Monit

Up-Regulated Maternal Embryonic Leucine Zipper Kinase Predicts Poor Prognosis of Hepatocellular Carcinoma Patients in a Chinese Han Population.

Authors - Shaohan Wu
View Article
humanIHC

Drug Des Devel Ther

A Novel Tetramethylpyrazine Chalcone Hybrid- HCTMPPK, as a Potential Anti-Lung Cancer Agent by Downregulating MELK

Authors - Yan Ma
View Article
humanWB,IHC

J Cancer

Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma

Authors - Yan Wang
View Article
humanWB,IHC

Cancer Cell Int

Upregulation of MELK promotes chemoresistance and induces macrophage M2 polarization via CSF-1/JAK2/STAT3 pathway in gastric cancer

Authors - Pengfei Su
View Article