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MED28 Polyclonal antibody

MED28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16262-1-AP

Synonyms

1500003D12Rik, EG1, Endothelial derived protein 1, magicin, MED28, mediator complex subunit 28, Tumor angiogenesis marker EG 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MED28 Polyclonal antibody (16262-1-AP)

    WB analysis using 16262-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using MED28 Polyclonal antibody (16262-1-AP)

    WB analysis of PC-12 using 16262-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of mouse testis using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of mouse testis using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human kidney using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human kidney using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human placenta using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human placenta using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human testis using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human testis using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human brain using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human brain using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human liver using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human liver using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human ovary using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of human ovary using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of mouse brain using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MED28 Polyclonal antibody (16262-1-AP)

    IHC staining of mouse brain using 16262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, PC-12 cells, rat testis tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human liver tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16262-1-AP targets MED28 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED28 fusion protein Ag9327
Full Name mediator complex subunit 28
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC011936
Gene Symbol MED28
Gene ID (NCBI) 80306
RRIDAB_2281863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MED28 antibody 16262-1-APDownload protocol
IHC protocol for MED28 antibody 16262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols