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- KD/KO Validated
MED12 Polyclonal antibody
MED12 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 20028-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
Product Information
20028-1-AP targets MED12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | mediator complex subunit 12 |
| Calculated Molecular Weight | 243 kDa |
| Observed Molecular Weight | 243 kDa |
| GenBank Accession Number | NM_005120 |
| Gene Symbol | MED12 |
| Gene ID (NCBI) | 9968 |
| RRID | AB_10644141 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED12 antibody 20028-1-AP | Download protocol |
| IF protocol for MED12 antibody 20028-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission. | ||
Mod Pathol MED12 and HMGA2 mutations: two independent genetic events in uterine leiomyoma and leiomyosarcoma. | ||
Genes (Basel) MED12 Regulates Smooth Muscle Cell Functions and Participates in the Development of Aortic Dissection.
| ||
Pathol Res Pract Cyclin-dependent kinase 8 is an independent prognosticator in uterine leiomyosarcoma. | ||
Naunyn Schmiedebergs Arch Pharmacol Loureirin B improves H/R-induced hepatic ischemia-reperfusion injury by downregulating ALOX5 to regulate mitochondrial homeostasis |
